Obsectives:
@EN In order to investigate the best cooling-thawing method, according to cell stage and cryoprotectants.
@ES Material & Method :
@EN 2-cell and 4-cell stage of mouse embryos were cryopreved by slow, rapid and ultrarapid cooling method using the Propanediol and Dimethyl Sulfoxide, each other. After than, the cyopreserved embryos were thawed by rapid thawing method. The
recovered
embryos, developed embryos were observed and compared among three cooling methods.
@ES Result: The results were as follows:
@EN 1. When 2-cell mouse embryos with Propanediol were cyropserved by Slow cooling-Rapid thawing method, the cleavage rate(84.0%) and the hatching rate (43.3%) are significantly higher than the other methods.
2. When 2-cell mouse embryos with DMSO were cryopreserved by Rapid cooling-Ra-pid thawing method, the cleavage rate(84.2%0 and the hatching rate (54.8%) are significantly higher than the other methods.
3. When 4-cell mouse embryos with Propanediol were cryopreserved by Slow cooling-Rapid thawing method, the cleavage rate (74.0%) and hatching rate (54.8%) are significantly higher than the other methods.
4. When 4-cell mouseembryos with DMSO were cryopreserved by Rapid colling-Rapid thawing method, the cleavage rate(84.4%) and the hatching rate (43.8%) are significantly higher than the other methods.
@ES Conclusion:
@EN We concluded that the mostly adequate cryopreserved method of 2-cell and 4-cell stage of mouse embryos using propanediol is the slow cooling-rapid thawing method and using DMSO is rapid cooling-rapid thawing method.
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